蛋奶中含有丰富的脂肪、蛋白质和钙、铁等营养成分，对人体健康至关重要。因此，我们需要采用相关检测技术对蛋奶制品进行质量监控。
荧光分光光度法具有高灵敏度，前处理简单，数据采集便利，光谱信息含量丰富的特征，本文主要介绍使用荧光分光光度计测量不同蛋奶制品的实验案例。
mso-fareast-font-family:宋体">2. 蛋奶制品的荧光测量
mso-fareast-font-family:宋体">2.1 牛奶的荧光光谱
由于牛奶是不透明的液体，通过使用固体样品支架和比色皿，测量牛奶的表面荧光，可以准确获得未稀释牛奶的荧光特性。
mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;mso-bidi-language:AR-SA">样品：牛奶
mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;mso-bidi-language:AR-SA">结果与讨论
mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;mso-bidi-language:AR-SA">
mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;mso-bidi-language:AR-SA">图 mso-fareast-font-family:宋体;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN; mso-bidi-language:AR-SA">1 mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-US;mso-fareast-language: ZH-CN;mso-bidi-language:AR-SA"> mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-US;mso-fareast-language: ZH-CN;mso-bidi-language:AR-SA">牛奶溶液的测量状态
图 mso-fareast-font-family:宋体">2 牛奶的三维荧光光谱（未稀释）
mso-hansi-font-family:"Times New Roman"">从图 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">2的三维荧光光谱中，确定了（ mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">i） mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">~(iv)四个荧光指纹峰，其中激发波长位于 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">280nm、发射波长位于 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">340nm的荧光指纹 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">(i)是源自蛋白质的氨基酸峰，通过此特征峰来判别牛奶的质量，是否为高蛋白牛奶。 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">
mso-hansi-font-family:"Times New Roman"">另外，由于储存在容器中的牛奶容易残留在容器壁上，这次我们通过稀释牛奶，模拟洗涤牛奶容器时的漂洗水。浓度为 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">1mg/L的牛奶溶液和纯净水的三维荧光光谱如图 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">3和图 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">4所示。两者对比，可以确定牛奶溶液中特征峰 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">(i)的位置。 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">
mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-US;mso-fareast-language: ZH-CN;mso-bidi-language:AR-SA">
"Times New Roman";mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font: minor-bidi;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;mso-bidi-language: AR-SA">图 mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-US;mso-fareast-language: ZH-CN;mso-bidi-language:AR-SA">3 1mg/L mso-hansi-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-US;mso-fareast-language: ZH-CN;mso-bidi-language:AR-SA">牛奶的三维荧光光谱
"Times New Roman";mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font: minor-bidi;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;mso-bidi-language: AR-SA">图 mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-US;mso-fareast-language: ZH-CN;mso-bidi-language:AR-SA">4 mso-hansi-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-US;mso-fareast-language: ZH-CN;mso-bidi-language:AR-SA">纯净水的三维荧光光谱
固定激发波长 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">280nm，分别测量了浓度范围在 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">0.1~1mg/L的牛奶样品和 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">0.1~10mg/L的牛奶样品在此激发波长处的荧光光谱，将得到的谱图进行叠加，得到图 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">5和图 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">6。
"Times New Roman";mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font: minor-bidi;mso-ansi-language:EN-US;mso-fareast-language:ZH-CN;mso-bidi-language: AR-SA">图 mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-US;mso-fareast-language: ZH-CN;mso-bidi-language:AR-SA">5 mso-hansi-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-US;mso-fareast-language: ZH-CN;mso-bidi-language:AR-SA">稀牛奶溶液（ mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi;mso-ansi-language: EN-US;mso-fareast-language:ZH-CN;mso-bidi-language:AR-SA">0.1~10mg/L "Times New Roman";mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-US; mso-fareast-language:ZH-CN;mso-bidi-language:AR-SA">）的荧光光谱
"Times New Roman"">图 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">6 "Times New Roman"">低浓度牛奶溶液（ mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">0.1~1mg/L "Times New Roman"">）的荧光光谱
mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">
mso-hansi-font-family:"Times New Roman"">通过比较不同浓度下牛奶溶液的荧光光谱，可以看到即使牛奶溶液的浓度为 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">0.1mg/L，通过荧光分光光度计也可以获取到荧光信号。因此通过测定牛奶容器漂洗水的荧光光谱，能够确认容器是否清洗干净。
除了牛奶之外，鸡蛋、奶粉等的荧光分析法请点击：蛋奶制品的荧光分析检测仪器_检测方案_日立高新技术公司 (instrument.com.cn)
mso-fareast-font-family:"Times New Roman"">3. 总结 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">
mso-hansi-font-family:"Times New Roman"">在蛋奶制品中，对于不透明的样品溶液和固体粉末，可以通过使用固体样品支架和荧光分光光度计实现样品表面荧光的测量。日立荧光分光光度计 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">F-7100具有超高扫描速度和高灵敏度，无需复杂的样品前处理，可以快速无损的获取蛋奶制品的荧光光谱。 mso-fareast-font-family:宋体;mso-bidi-theme-font:minor-bidi">